Incorporation of Sulfate into the Capsular Polysaccharide of the Red Alga Porphyridium

The accumulation of sulfate-(35)S by Porphyridium aerugineum cells and subsequent appearance of solubilized capsular polysaccharide-(35)S in the growth medium were examined The uptake of label by the cells was largely light dependent. Pulse-chase experiments using log phase cells revealed a rapid labeling of solubilized capsular polysaccharide, recovered from the medium as the cetylpyridinium chloride precipitate Polyacrylamide gel electrophoresis of the polysaccharide-(35)S showed the sulfate to be firmly bound to an immobile fraction. Sephadex chromatography revealed the molecular weight of the polysaccharide to be in excess of 2 x 10(5). Acid hydrolysis of the polysaccharide-(35)S released sulfate-(35)S ion as evidenced by radioautography of thin layer chromatographs Preliminary electron microscope evidence suggests that the synthesis, movement, and deposition of the capsular polysaccharide on the cell surface are Golgi complex-mediated processes